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here one that codes for a region of nonstructural hydrophilic protein, NS-3. Computer analysis of this sequence (967 bp) showed about 87% conservation at the amino acid level and 79% at the nucleotide level when compared with dengue serotypes 2, 3 and 4 and Japanese encephalitis virus. This suggests an important function which is common to all four serotypes. Comparison of the cloned region with sequences of the above strains also suggested conservation of hydrophobic regions.

Barkan, H. (1919). "The ocular complications of dengue fever." Am.J.Ophthalmol. 2: 650-652.

Barnes, W. J. S. and L. Rosen (1974). "Fatal hemorrhagic disease and shock associated with primary dengue infection on a Pacific island." Am.J.Trop.Med.Hyg. 23(3): 495-506.

Barrett, A. D., J. H. Mathews, et al. (1990). "Identification of monoclonal antibodies that distinguish between 17D-204 and other strains of yellow fever virus." J.Gen.Virol. 71: 13-18.
Eight monoclonal antibodies (MAbs) prepared against the flaviviruses Saint Louis encephalitis, dengue 2 and dengue 3 viruses all recognized epitopes on the envelope protein of the prototype flavivirus, yellow fever (YF) virus. Three of these MAbs with flavivirus group-common specificity and two MAbs with a flavivirus-subgroup specificity were found to distinguish wild- type YF viruses from YF 17D-204 vaccine virus, but not from the closely related 17DD vaccine virus, nor from the French neurotropic vaccine virus. This pattern of reactivity was seen only with viruses grown in Aedes albopictus C6/36 cells and not with viruses grown in vertebrate cells (SW13 and Vero cells), where all five MAbs recognized epitopes on both wild-type and 17D- 204 viruses. Examination of adult A. aegypti mosquitoes infected with the same YF viruses as above gave a different pattern of results to those in C6/36 cells. Thus, epitope expression differs between mammalian and arthropod cells and between arthropod cells in vitro and in vivo. Neutralization tests showed that all five MAbs would neutralize wild-type Asibi virus grown in SW13 cells, but not Asibi virus grown in C6/36 cells, nor 17D-204 vaccine virus grown in either cell type. Therefore, it is concluded that when YF virus is grown in mosquito cells, wild-type virus is antigenically and biologically distinct from the 17D-204 vaccine virus.

Barrett, A. D. (1997). "Japanese encephalitis and dengue vaccines." Biologicals 25(1): 27-34.

Barth, O. M. (1992). "Replication of Dengue Viruses in Mosquito Cell Cultures - A Model from Ultrastructural Observations." Mem.Inst.Oswaldo.Cruz. (Rio de Janeiro) 87: 565-574.
Mosquito cell cultures infected with human sera from dengue-1 and dengue-2 outbreaks, started in Rio de Janeiro by 1986 and 1990 respectively, were examined by electron microscopy at different times post the infection of cell cultures. More information was obtained about cell penetration of virus particles in the presence or not of antibodies, their pathway inside the cells, replication mode and exit. Infectiveness of the virus at those different stages. can only be attributed to the particles appearing inside