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the trans- Golgi vesicles; most of all newly formed virus particles remain inside the RER-derived cell vesicles or inside lysosomes, even during cell lysis. Groups of larger particles, 65-75 nm in diameter at dengue-2 infections, persist during cell passage. The large amounts of smooth membrane structures, as vesicles or tubules inside the RER, are attributed to a cell response to viral infection

Barth, O. M., L. M. D. Cortes, et al. (1994). "Ultrastructural aspects of virus replication in one fatal case and several other isolates from a dengue type 2 outbreak in Rio de Janeiro." Mem.Inst.Oswaldo.Cruz. (Rio de Janeiro) 89: 21-24.
OM Barth, Inst Oswaldo Cruz, Dept Virol, AV Brasil 4365, BR- 21045900 Rio Janeiro, Brazil Dengue virus replication in mosquito cell cultures was observed by electron microscopy in one fatal and 40 classical isolates from a dengue type 2 outbreak in Rio de Janeiro and compared with the prototype New Guinea C strain. All the Br azilian isolates presented beside the classical structured dengue virus particles, fuzzy coated virus-like particles, never observed in the referencial New Guinea C virus strain. More numerous DEN- 2 virus particles, fuzzy coated virus-like particles, defective virus particles and smooth membrane structures inside the rough endoplasmic reticulum characterized the unique fatal isolate examined

Barth, O. M., L. M. Cortes, et al. (1996). "Ultrastructural aspects of the replication of dengue virus type 2 isolated in Brazil." Mem.Inst.Oswaldo.Cruz. (Rio de Janeiro) 91: 255-256.

Bartholomeusz, A. I. and P. J. Wright (1993). "Synthesis of Dengue Virus RNA Invitro - Initiation and the Involvement of Proteins NS3 and NS5." Arch.Virol. 128: 111-121.
An assay for flavivirus RNA-dependent RNA polymerase activity in vitro was established using extracts of Vero cells infected with dengue virus type 2 (DEN-2) or Kunjin virus (KUN). RNA synthesis was initiated on a template of viral replicative form (RF) and RF was converted to the replicative intermediate (RI). The RNA- dependent RNA polymerase complex of DEN-2 utilised either DEN-2 or KUN RF as template, and similarly the KUN polymerase complex utilised either DEN-2 or KUN RF template. In addition, antibodies against the nonstructural proteins NS3 and NS5 inhibited the conversion of RF to RI, indicating that NS3 and NS5 are involved in viral RNA replication

Bartholomeusz, A., E. Tomlinson, et al. (1994). "Use of a flavivirus RNA-dependent RNA polymerase assay to investigate the antiviral activity of selected compounds." Antiviral Res. 24: 341-350.
A Bartholomeusz, Fairfield Hosp, Macfarlane Burnet Ctr, Yarra Bend Rd, Fairfield, Vic 3078, Australia We have developed an assay using flavivirus RNA- dependent RNA polymerase to test the inhibitory activity of potential antiviral agents. The effects of antiviral agents on RNA. synthesis were examined in this assay using extracts of Vero cells infected with dengue virus type 2 or Kunjin virus. Several different classes of known polymerase inhibitors were tested. The synthesis of double- stranded replicative form RNA was inhibited in a dose-dependent fashion in the presence of the polyoxometalate HPA-23 [(NH4)(18)(NaW21Sb9O86)(17)].14 H2O and several